Proliferation of Canine Intervertebral Disk Chondrocytes in Three-Dimensional Alginate Microsphere Culture.
نویسندگان
چکیده
منابع مشابه
Costal Versus Articular Chondrocytes in Alginate Three-Dimensional Cultures
Given the difficulties in accessing articular cartilage as a source of chondrocytes to be used in fabricating cartilage constructs, alternative sources are required. The present study examined chondrocytes from costal cartilage for their suitability in cartilage tissue engineering. Chondrocytes isolated from rat knee and rib hyaline cartilage were separately mixed with alginate and placed in a ...
متن کاملQuantitative Analysis of the Proliferation and Differentiation of Rat Articular Chondrocytes in Alginate 3D Culture
Background: While articular chondrocytes are among those appropriate candidates for cartilage regeneration, the cell dedifferentiation during monolayer culture has limited their application. Several investigations have indicated the usefulness of alginate, but the topic of proliferation and differentiation of chondrocytes in alginate culture has still remained controversial. Methods: Rat articu...
متن کاملcostal versus articular chondrocytes in alginate three-dimensional cultures
given the difficulties in accessing articular cartilage as a source of chondrocytes to be used in fabricating cartilage constructs, alternative sources are required. the present study examined chondrocytes from costal cartilage for their suitability in cartilage tissue engineering. chondrocytes isolated from rat knee and rib hyaline cartilage were separately mixed with alginate and placed in a ...
متن کاملquantitative analysis of the proliferation and differentiation of rat articular chondrocytes in alginate 3d culture
background: while articular chondrocytes are among those appropriate candidates for cartilage regeneration, the cell dedifferentiation during monolayer culture has limited their application. several investigations have indicated the usefulness of alginate, but the topic of proliferation and differentiation of chondrocytes in alginate culture has still remained controversial. methods: rat articu...
متن کاملCulture of chondrocytes in alginate beads.
A classic method for the encapsulation and culture of chondrocytes in alginate beads is described. Chondrocytes are released from cartilage matrix by collagenase/dispase digestion and mixed with a solution of 1.25% alginic acid until a homogenous suspension is obtained. The suspension is drawn into a syringe and pushed gently through a needle, so that drops fall into a solution of calcium chlor...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2002
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.64.79